Which type of electrochemical biosensor is urea biosensor?
For the electrochemical measurement of the concentration of urea, a urease-based enzyme biosensor has been developed [13] . Urease is a nickel-containing metalloenzyme found in various bacteria, fungi, algae, and plants [14]. …
What is immobilization in biosensor?
Biosensors that use immobilized enzymes belong to a catalytic type of biosensor in which the transducer surface is immobilized with enzymes that act as a bridge between the transducer and the analyte.
What is a urea sensor?
The urease enzyme is used for the determination of urea, the urea sensor means an accurate determination of urea essential with respect to renal function and biomedical applications. ISE is a good approach to build chemical sensors in detecting urea analyte.
What is potentiometric device?
A potentiometric sensor is a type of chemical sensor that may be used to determine the analytical concentration of some components of the analyte gas or solution. These sensors measure the electrical potential of an electrode when no current is present.
Which statement about enzyme is true?
This statement about enzymes is true: Enzymes accelerate reactions by lowering the activation energy. Enzymes are proteins whose three-dimensional form is key to their function. Enzymes do not alter the overall change in free energy for a reaction.
What is enzyme based biosensor?
An enzymatic biosensor comprises of an enzyme, which recognizes and then reacts with the target analyte producing a chemical signal, a transducer, which produces a physical signal out of that chemical one, and an electronic amplifier, which conditions and then amplifies the signal.
Which are the enzyme based membrane electrode used to determine urea?
A pH-membrane electrode with n-tridodecylamine (TDDA) as the hydrogen-ion-selective ionophore was used for the construction of a potentiometric biosensor for urea determination. The electrode was enzymatically modified by covalent binding of urease molecules directly to the surface of the potentiometric membrane.