What is mate pair sequencing?

What is mate pair sequencing?

Mate pair sequencing involves generating long-insert paired-end DNA libraries useful for a number of sequencing applications, including: De novo sequencing. Genome finishing. Structural variant detection. Identification of complex genomic rearrangements.

What is paired end and mate pair sequencing?

Paired-end or mate-pair Follow In mate-pair sequencing, the library preparation yields two fragments that are distal to each other in the genome and in the opposite in orientation to that of a mate-paired fragment.

Why is paired end sequencing better?

Paired-end reading improves the ability to identify the relative positions of various reads in the genome, making it much more effective than single-end reading in resolving structural rearrangements such as gene insertions, deletions, or inversions. It can also improve the assembly of repetitive regions.

What is genome finishing?

Genome finishing is achieved by converting a set of contigs or scaffolds into complete sequences that represent the full genomic content of the organism without unknown regions (gaps) (Mardis et al., 2002), and aims a “closed” and full representation of the chromosome organization.

Why is paired-end sequencing better?

How do paired-end reads work?

The term ‘paired ends’ refers to the two ends of the same DNA molecule. So you can sequence one end, then turn it around and sequence the other end. The two sequences you get are ‘paired end reads’.

What are the applications of metamate pair sequencing?

Mate pair sequencing is used for various applications applications, including. De novo genome sequencing. Genome finishing. Structural variant detection. Identification of complex genomic rearrangements.

What is the advantage of using paired-end sequencing with mate pair libraries?

Combining data generated from mate pair library sequencing with that from short-insert paired-end reads provides a powerful combination of read lengths for maximal sequencing coverage across the genome. Using a combination of short and long insert sizes with paired-end sequencing results in maximal coverage of the genome for de novo assembly.

What is a “mate pair” library?

The preparation of “mate pair” libraries is designed to allow classical “paired-end” sequencing of both ends of a fragment with an original size of several kilobases. The figure shows the workflow for “mate-pair” library preparation for Illumina sequencing.

What is “mate-pair” library preparation for Illumina sequencing?

The figure shows the workflow for “mate-pair” library preparation for Illumina sequencing. Since the beginning of 2013, this preparation has been based on Nextera technology. The enzyme tagmentase fragments the DNA to sizes between 2 and 15 kb and binds adapters at the breaking point.