What is a Jurkat cell line?
Jurkat cells are an immortalized line of T lymphocyte cells. And they are used to study acute T cell leukemia, T cell signaling, and the expression of a number of chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science due to their ability to produce interleukin 2.
Are Jurkat cells CD4 positive?
Jurkat is a human CD4+-T-cell line that expresses CD3 along with a number of other T-cell surface receptors which are typical of freshly isolated CD4+ human peripheral blood T cells.
How do you split Jurkat cells?
Splitting can be performed by centrifuging cells at 500g for 5 minutes, decanting growth medium and rinsing in sterile 1X PBS. Cells should then be resuspended in fresh growth medium to achieve a density 1×10^5 and 1×10^6 cells/ml.
Is Jurkat CD4 or CD8?
Jurkat is a CD4+/CD8- T-cell lymphoma that expresses an endogenous TCR. The T2 line is a TAP-deficient T-cell/B-cell hybridoma expressing HLA-A2.
Is Jurkat a CD4?
Jurkat is a CD4 T cell line provided by O. Acuto (Institut Pasteur, Paris, France). Kit 225 is a CD4 T cell line expressing high- and intermediate-affinity IL-2R (13).
Are Jurkat cells suspension or adherent?
Jurkat Clone E6-1 is a human T lymphoblastoid cell line derived from an acute T cell leukemia. The cells are suspension lymphoblasts.
How do you culture a Jurkat cell?
Add 2 ml of the Jurkat cell suspension to a new sterile T75 flask containing 20 ml of complete growth medium and a final concentration of 1 mg/ml G418. For 75 cm2 flasks, use approximately 105 cells/flask and subculture every 3-4 days. Place the culture vessel in a 5% CO2, 37°C incubator.
Do Jurkat cells express CD4?
All Answers (1) The Jurkat T cell leukemic line E6–1 expresses high levels of mature TCR and low levels of CD4, but no CD8 or MHC class II.
Are Jurkat cells cytotoxic?
There are cytotoxic CD4 T cells. But if the CD4 T cell line used is the Jurkat T cell, is it possible to measure the cells’ cytotoxicity against their target cells (these Jurkat T cells have been modified to recognize certain tumor surface Ag?)
Do Jurkat cells express CD8?
Are Jurkat cells suspension cells?
Are Jurkat cells CD4 or CD8 positive?
What are Jurkat cells used for?
Jurkat cells are an immortalized line of T lymphocyte cells. And they are used to study acute T cell leukemia, T cell signaling, and the expression of a number of chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science due to their ability to produce interleukin 2.
Can Jurkat cells be co-culture with HepG2 cells?
A co-culture system of adherent HepG2 and suspension Jurkat cells was established. Approximately 5×103HePG2 cells were seeded onto a 96-well plate and treated with 10 ng/mL IFN-γ for 24 h. Activated Jurkat cells (5×104cells/well) were added into HepG2 cells at an E/T ratio of 10:1.
What is the stimulating concentration of PHA in co-culture of Jurkat cells?
Therefore, the stimulating concentration of PHA was set at 2 μg/mL in the cell co-culture system. Co-Culture with HePG2 Cells Induced PD-1 Overexpression on Jurkat Cells
What is the karyotype of a Jurkat cell line?
Jurkat (ATCC number TIB-152) cell culture and formaldehyde cross- linking Jurkat Clone E6-1 is a human T lymphoblastoid cell line derived from an acute T cell leukemia. The cells are suspension lymphoblasts. The karyotype is pseudodiploid human male cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at 5.3%.
1 Model systems. The Jurkat cell line is an immortalized T lymphocyte cell line that was originally obtained from the peripheral blood of a boy with T cell leukemia [14]. 2 Volume 2. Benjamin G. Neel. 3 Protein Prenylation Part B. E. Anouk Stigter. 4 Thymoquinone
How do I get a certificate of analysis for Jurkat (tib-152)?
To download a certificate of analysis for Jurkat, Clone E6-1 ( TIB-152 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of Jurkat, Clone E6-1 ( TIB-152) is not currently available online.
Is the Jurkat-FHCRC cell line Mycoplasma free?
The line was cloned from cells obtained from Dr. Kendall Smith and are mycoplasma free. This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line.
How many Jurkat and T cell activation publications are there?
A PubMed search using the terms ‘Jurkat’ and ‘T cell activation’ yielded over 6000 publications.